Immobilized Metal Affinity Chromatography (IMAC) for Metalloproteomics and Phosphoproteomics
| Autor: | YY Chang, Hongzhe Sun, Hongyan Li |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Chromatography Chemistry Metal ions in aqueous solution Phosphoproteomics 010402 general chemistry 01 natural sciences 0104 chemical sciences 03 medical and health sciences Human health 030104 developmental biology Biochemistry Metal affinity chromatography Metalloprotein Protein phosphorylation Phosphorylated proteins |
| DOI: | 10.1016/b978-0-12-803814-7.00009-5 |
| Popis: | Although metalloproteins or metal-binding proteins have been ascertained to be associated with human health and diseases, the roles of metalloproteins in disease processes are not fully understood and require further comprehensive exploration. Immobilized metal affinity chromatography (IMAC) was initially developed for the purification of polyhistidine-tagged proteins, and subsequently for the analysis of phosphorylated proteins. Upon incorporation of different metal ions [e.g., Co(II), Cu(II)] onto solid resin, IMAC was further extended to track or enrich metal-binding proteins to elucidate their biological processes in various prokaryotic and eukaryotic cells. This chapter summarizes the progress of IMAC in metalloproteomics and phosphoproteomics, providing deeper insights into the mode of action of essential metal ions and metal-based drugs as well as the role of phosphorylated proteins in multiple cellular processes. |
| Databáze: | OpenAIRE |
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