Meiotic transmission of T-DNA genes inArabidopsis thaliana plants and their expression after 5-azacytidine treatment

Autor:	Daniela Pavingerová, M. Hrouda
Rok vydání:	1991
Předmět:	Octopine Genetics food and beverages Opine Plant Science Horticulture Biology biology.organism_classification chemistry.chemical_compound Ti plasmid Biochemistry chemistry Biosynthesis DNA methylation Arabidopsis thaliana Gene DNA
Zdroj:	Biologia plantarum. 33
ISSN:	1573-8264 0006-3134
DOI:	10.1007/bf02897720
Popis:	Arabidopsis thaliana tumors were induced by octopine strain ofAgrobacterium tumefaciens B6S3 and its derivatives with modified T-DNA. Flowering shoots appeared sponta-neously onin vitro cultivated tumors and set seeds. R1 and R2 progeny of octopine synthesizing plants segregated in opine synthesis activities 3:1 and 15:1. Octopine synthase activity showed absolute linkage with agropine synthesis in most lines. In R3 and R4 progenies, the fraction of octopine synthase and agropine synthesis positive plants was lower than expected, but Mendelian segregation was restored if plants were cultivated on medium with 5-azacytidine. The most probable mechanism of disapearance of opine synthesis is cytosine methylation. The effect of 5-azacytidine lasted for at least next two generations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ff6f51360360dce5d6c2aae60fb6e569 https://doi.org/10.1007/bf02897720 Zobrazit plný text záznamu Full text from SpringerLink