Effect of calmodulin and voltage-dependent Ca2+ channel on the proliferation of heptoma cells induced by epidermal growth factor

Autor:	Wang Jialong, Cui Wuren, Wu Binwen, Yuan Shunyu
Rok vydání:	2003
Předmět:	Calmodulin biology DNA synthesis Kinase Chemistry Biomedical Engineering Biochemistry Molecular biology digestive system diseases Cell biology Biomaterials Epidermal growth factor Mole Genetics biology.protein Ca2 channels Inhibitory effect Tyrosine kinase Earth-Surface Processes
Zdroj:	Journal of Huazhong University of Science and Technology [Medical Sciences]. 23:26-28
ISSN:	1993-1352 1672-0733
DOI:	10.1007/bf02829454
Popis:	The effect of thyrosine kinase, calmodulin and voltage-dependent Ca2+ channel on the proliferation of hepatoma cells induced by EGF was studied. Hepatoma cell line SMMC7721 was cultured in RPMI1640 serum-free medium. DNA synthesis rate of hepatoma cells was measured by H-TdR incorporation. 10−9 mol/L EGF could significantly stimulate the proliferation of hepatoma cells (P 0.05), but it had very significantly inhibitory effect on the proliferation of hepatoma cells induced by EGF (P 0.05). It had no effect on the basic phase of cultured hepatoma cells, (P>0.05). It is suggested that tyrosine kinase and Ca2+-calmodulin-dependent pathway may play a critical role on the proliferation of heptoma cells induced by EGF and voltage-dependent Ca2+ channel is independent of the effect of EGF.
Databáze:	OpenAIRE
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