Transcriptome sequencing of PBMC reveals Hsa-miR-124-5p regulates cell cycle and metabolism of lipids via targeting CSTF2/ TXNRD1 in hearing loss patients

Autor: Dongdong Zhang, Yixin Sun, Cong Zou, Yongjun Hong, Min Lei, Yue Wang, Peiji Zeng, Wei Wang, Fangyu Qi, Chengfu Cai
Rok vydání: 2022
DOI: 10.21203/rs.3.rs-1439086/v1
Popis: BackgroundHearing loss (HL) is a public health event, which seriously affects the happiness of people’s life. Hsa-miR-124-5p has not been reported in HL. This study aimed to construct a miRNA-mRNA network associated with HL.MethodsSubjects were screened through clinical audiology experiments. MiRNA-seq was performed on the peripheral blood mononuclear cells (PBMC), and the differentially expressed miRNAs (DEMs, P-Value < 0.05, ∣log FC | ≥1) were obtained by analysis. We selected the most significantly up-regulated DEMs for research. We predicted the downstream target genes of the most significantly up-regulated DEMs through miRDB, mirDIP, mirtarbase and TargetScan databases, and four database overlapping genes were considered as downstream target genes. Enrichment analysis was performed on overlapping genes. Then, a protein-protein interaction (PPI) network of overlapping genes was performed through STRING database, aimed to pick out hub genes. Finally, we construct the miRNA-mRNA network.ResultsThere were 6 clinical participants, 3 hearing loss patients and 3 healthy subjects.There are 29 DEMs, of which hsa-miR-124-5p is the most significantly up-regulated DEMs. 13 downstream target genes corresponding to hsa-miR-124-5p were screened, which were significantly enriched in cell cycle and metabolism of lipids. PPI analysis was performed on 13 target genes, and two hub genes (CSTF2/ TXNRD1) were finally obtained. Most importantly, miRNA-mRNA networks containing hsa-miR-124-5p/CSTF2 and hsa-miR-124-5p/TXNRD1 were successfully constructed.ConclusionsIn summary, hsa-miR-124-5p may be a novel biomarker for hearing loss patients and play a important role in hearing loss patients by targeting CSTF2 and TXNRD1. Hsa-miR-124-5p may participate in cell cycle and metabolism of lipids biological process in hearing loss patients.
Databáze: OpenAIRE