Module for multiphoton high-resolution hyperspectral imaging and spectroscopy
| Autor: | Tommaso Baldacchini, Aram Zeytunyan, Ruben Zadoyan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Materials science
business.industry Physics::Optics Second-harmonic generation Hyperspectral imaging 02 engineering and technology Grating 021001 nanoscience & nanotechnology Laser 01 natural sciences law.invention 010309 optics symbols.namesake Optics law 0103 physical sciences Microscopy symbols Physics::Atomic Physics Spectral resolution 0210 nano-technology Raman spectroscopy Spectroscopy business |
| Zdroj: | Multiphoton Microscopy in the Biomedical Sciences XVIII. |
| DOI: | 10.1117/12.2287943 |
| Popis: | We developed a module for dual-output, dual-wavelength lasers that facilitates multiphoton imaging and spectroscopy experiments and enables hyperspectral imaging with spectral resolution up to 5 cm−1. High spectral resolution is achieved by employing spectral focusing. Specifically, two sets of grating pairs are used to control the chirps in each laser beam. In contrast with the approach that uses fixed-length glass rods, grating pairs allow matching the spectral resolution and the linewidths of the Raman lines of interest. To demonstrate the performance of the module, we report the results of spectral focusing CARS and SRS microscopy experiments for various test samples and Raman shifts. The developed module can be used for a variety of multimodal imaging and spectroscopy applications, such as single- and multi-color two-photon fluorescence, second harmonic generation, third harmonic generation, pump-probe, transient absorption, and others. |
| Databáze: | OpenAIRE |
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