Electrochemistry and electrocatalytic properties of mixed assemblies of horseradish peroxidase, poly(diallyl dimethylammonium chloride) and gold nanoparticles on a glassy carbon electrode

Autor:	Zongrang Zhang, Xiaoling Ma, Guoping Duan, Haifeng Yang, Wei Ren, Ying Wen, Yao Wang
Rok vydání:	2009
Předmět:	biology Chemistry Inorganic chemistry Electrocatalyst Electrochemistry Horseradish peroxidase Analytical Chemistry Dielectric spectroscopy Electron transfer Chemical engineering Colloidal gold biology.protein Cyclic voltammetry Biosensor
Zdroj:	Microchimica Acta. 166:283-288
ISSN:	1436-5073 0026-3672
DOI:	10.1007/s00604-009-0202-y
Popis:	Poly(diallyldimethylammonium chloride), gold nanoparticles (Au-NPs; 2–5 nm) and horseradish peroxidase (HRP) were alternately self-assembled on a glassy carbon electrode. Due to the conducting wire effect of the Au-NPs, the HRP undergoes direct electrochemistry with a small peak-to-peak separation of 33 mV and a formal potential of −0.370 V (versus SCE). The reaction involves a single electron transfer coupled to a one-proton transfer reaction. Electrochemical impedance spectroscopy and cyclic voltammetry also were applied to characterize the self-assembly process and to study the electrochemical behavior of the immobilized HRP. Its good electrocatalytic response to the reduction of hydrogen peroxide resulted in a novel third-generation biosensor for this species.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ff4bb1a074dfbdbd53f21d471e0df20d https://doi.org/10.1007/s00604-009-0202-y Zobrazit plný text záznamu Plný text ve formátu PDF