| Autor: |
Jianjun Miao, Trevor J. Simmons, Robert J. Linhardt, Minoru Miyauchi, Jonathan S. Dordick |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Journal of Chromatography & Separation Techniques. |
| ISSN: |
2157-7064 |
| DOI: |
10.4172/2157-7064.1000110 |
| Popis: |
Flexible, well-dispersed and continuous 100-nm diameter cellulose fibers were prepared from an ionic liquid solvent by a novel dry-jet wet-electro spinning process. The ribbon fibers formed were chemically activated and an affinity dye, cibacron blue (CB) was immobilized at 0.22 g CB/g dry fibers loading to the surface of these fibers. The resulting affinity matrix was packed into a chromatography column and the adsorption, desorption and specificity of this matrix for bovine serum albumin (BSA) was studied. These electrospun fibers had a BSA binding capacity of 230 mg/g, nearly twice that of CB-immobilized 100-?m beads and over ten-fold higher capacity that CB-immobilized cellulose fibers prepared by a conventional electrospinning process. The results of this work suggest that chromatography supports of flexible, well-dispersed, continuous nanofibers may offer advantages over conventional supports in affinity separations. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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