Extraction of total RNA from E. coli cells v1
Autor: | Alice Pawlowski |
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Rok vydání: | 2016 |
Předmět: | |
ISSN: | 0003-2697 |
Popis: | The protocol is used for the extraction of total RNA from E. coli cells. It is based on the method described by Chomczynski and Sacchi, 1987 (https://doi.org/10.1016/0003-2697(87)90021-2). Total RNA is isolated from cells expressing small regulatory RNAs (trRNAs) either constitutively (E. coli W3110) or after induction with anhydrotetracycline (E. coli W1310 Z1). |
Databáze: | OpenAIRE |
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