Extraction of total RNA from E. coli cells v1

Autor: Alice Pawlowski
Rok vydání: 2016
Předmět:
ISSN: 0003-2697
Popis: The protocol is used for the extraction of total RNA from E. coli cells. It is based on the method described by Chomczynski and Sacchi, 1987 (https://doi.org/10.1016/0003-2697(87)90021-2). Total RNA is isolated from cells expressing small regulatory RNAs (trRNAs) either constitutively (E. coli W3110) or after induction with anhydrotetracycline (E. coli W1310 Z1).
Databáze: OpenAIRE