| Autor: |
Megha Pande, S.K. Ghosh, S. Tyagi, R. Katiyar, N. Srivastava, M. Karikalan, S. Kumar, K. Krishnappa, A.S. Sirohi, null Sarika, A. Mitra |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Cryoletters. 43:276-282 |
| ISSN: |
0143-2044 |
| DOI: |
10.54680/fr22510110712 |
| Popis: |
BACKGROUND: Cryopreservation process negatively affects spermatozoa functions. Humanin, a small polypeptide encoded in the mitochondrial genome, is well known for its role in cell survival. OBJECTIVE: To quantify the endogenous levels of humanin in seminal plasma of crossbred Frieswal bulls and to study its role in cryoprotection. The presence of humanin in bull spermatozoa was also investigated. MATERIALS AND METHODS: A total of 40 semen samples were separated into two groups based on the initial progressive motility (IPM): Good (IPM ≥70%) and Poor ( IPM ≤50%) groups; and/or based on the post-thaw motility (PTM): Freezable (PTM≥50%) and Non-freezable (PTM < 50%) groups. Humanin concentration in seminal plasma (SP-HN) was quantified using ELISA. RESULTS: SP-HN concentration ranged from undetectable to 67.6 pg/mL with a median level of 35.2 pg/mL. SP-HN level was significantly higher in the good quality semen group than in the poor quality semen group (p CONCLUSION: Endogenous humanin level had significant correlation with semen quality and might protect sperm cells against freeze-induced oxidative stress. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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