Serum proteomic assessment of the progression of fracture healing
| Autor: | Paul Tornetta, Heather E. Matheny, Louis C. Gerstenfeld, Amira I. Hussein, Christian Mancini, Brenna L. Hogue, Margaret E. Cooke, Kyle Lybrand |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Microarray Microarray analysis techniques Mesenchymal stem cell Wnt signaling pathway 030208 emergency & critical care medicine Inflammation Bone healing Biology Cell biology Transcriptome 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Proteome medicine Orthopedics and Sports Medicine medicine.symptom |
| Zdroj: | Journal of Orthopaedic Research. |
| ISSN: | 0736-0266 |
| Popis: | A targeted proteomic analysis of murine serum over a 35-day course of fracture healing was carried out to determine if serum proteomic changes could be used to monitor the biological progression of fracture healing. Transverse, closed femoral fractures where generated and stabilized with intramedullary fixation. A single stranded DNA aptamer-based multiplexed proteomic approach was used to assay 1,310 proteins. The transcriptomic profiles for genes matching the 1,310 proteins were obtained by microarray analysis of callus mRNA. Of the 1,310 proteins analyzed, 850 proteins showed significant differences among the time points (p-value |
| Databáze: | OpenAIRE |
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