Essential role of CD4+ T cells for the activation of group 2 innate lymphoid cells during respiratory syncytial virus infection in mice

Autor:	Na Zhao, Beixing Liu, Wenwen Zhu, Xu Han, Yulin Cui, Song Bai
Rok vydání:	2019
Předmět:	Lung medicine.diagnostic_test medicine.drug_class Immunology Innate lymphoid cell Biology Monoclonal antibody Virus Flow cytometry medicine.anatomical_structure Real-time polymerase chain reaction Oncology medicine Immunology and Allergy Respiratory system
Zdroj:	Immunotherapy. 11:1303-1313
ISSN:	1750-7448 1750-743X
DOI:	10.2217/imt-2019-0084
Popis:	Aim: To investigate whether and how CD4+ T cells contribute to ILC2 activation during respiratory syncytial virus (RSV) infection. Methods: The methods of flow cytometry, quantitative PCR and ELISA were used in the present study. Results: Depletion of CD4+ T cells diminished the numbers of lung ILC2s as well as their ability to produce type 2 cytokines. CD4+ T cell-mediated ILC2 activation is related to IL-2. The main cellular source of IL-2 was CD4+ T cells. Depletion of CD4+ T cells decreased IL-2 levels in the lungs of RSV-infected mice. IL-2 can directly stimulate ILC2 proliferation and promote ILC2s to produce cytokines. Treatment of mice with neutralizing anti-IL-2 monoclonal antibodies diminished ILC2 activation. Conclusion: These results suggest that CD4+ T cells contribute to RSV-induced ILC2 activation partly via producing IL-2.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ff0aa5d0ed250cf4942ff15f37693378 https://doi.org/10.2217/imt-2019-0084 Zobrazit plný text záznamu