Fast multicolor 3D imaging using aberration-corrected multifocus microscopy
| Autor: | Sara Abrahamsson, Jan Wisniewski, Xavier Darzacq, Dugast Darzacq Claire, Cornelia I. Bargmann, Florian Mueller, Carl Wu, Pierre Soule, Bassam Hajj, Gaku Mizuguchi, Mats G. L. Gustafsson, Maxime Dahan, Jiji Chen, Alexander Y. Katsov, David A. Agard, Sjoerd Stallinga |
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| Rok vydání: | 2013 |
| Předmět: |
2d images
0303 health sciences Materials science business.industry Saccharomyces cerevisiae Proteins Cell Biology 01 natural sciences Biochemistry Cell biology 010309 optics 03 medical and health sciences Optics 0103 physical sciences Microscopy Fluorescence microscope Cell tracking Single image business Molecular Biology 030304 developmental biology Biotechnology |
| Zdroj: | Nature Methods. 10:60-63 |
| ISSN: | 1548-7105 1548-7091 |
| DOI: | 10.1038/nmeth.2277 |
| Popis: | Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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