| Popis: |
We have recently introduced a family of mild and efficient solid-phase reagents [1], derived from Ellman's reagent, to form disulfide bridges in peptides and protein under a wide range of conditions. Towards the same goal, two additional solid-phase reagents derived from -dithiobisnicotinic acid (1) and -dithiobis(5-nitronicotinic acid) (2) were designed, prepared, and investigated (Fig. 1). Using somatostatin (14 residues, disulfide bridge between residues 3 and 14) and differentially protected derivatives of SI (13 residues, the natural isomer has disulfide bridges between residues 2 and 7; 3 and 13) as substrates, the two reagents were evaluated under various reaction conditions. Their capacity to promote intramolecular disulfide bridge formation was compared to that of the solid-phase Ellman's reagent. |
