Current PCR-based methods for the detection of mycotoxigenic fungi in complex food and feed matrices
| Autor: | Qi Zhang, H. Ur Rahman, Peiwu Li, Q. Yu, Xiaofeng Yue, Wen Zhang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Fusarium
0303 health sciences Aspergillus 030306 microbiology business.industry Public Health Environmental and Occupational Health 04 agricultural and veterinary sciences Biology Toxicology biology.organism_classification Food safety 040401 food science 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology chemistry Penicillium Food science Mycotoxin business Food Science |
| Zdroj: | World Mycotoxin Journal. 13:139-150 |
| ISSN: | 1875-0796 1875-0710 |
| DOI: | 10.3920/wmj2019.2455 |
| Popis: | Mycotoxins are toxic secondary fungal metabolites produced by certain types of filamentous fungi, such as Aspergillus, Fusarium, and Penicillium spp. Mycotoxigenic fungi and their produced mycotoxins are considered to be an important issue in food and feed safety due to their toxic effects like carcinogenicity, immunosuppression, neurotoxicity, nephrotoxicity, and hepatotoxicity on humans and animals. To boost the safety level of food and feedstuff, detection and identification of toxins are essential at critical control points across food and feed chains. Zero-tolerance policies by the European Union and other organizations about the extreme low level of tolerance of mycotoxins contamination in food and feed matrices have led to an increasing interest to design more sensitive, specific, rapid, cost-effective, and safer to use mycotoxigenic fungi detection technologies. Hence, many mycotoxigenic fungi detection technologies have been applied to measure and control toxins contamination in food and feed substrates. PCR-based mycotoxigenic fungi detection technologies, such as conventional PCR, real-time PCR, nested PCR, reverse transcriptase (RT)-PCR, loop-mediated isothermal amplification (LAMP), in situ PCR, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR DGGE), co-operational PCR, multiplex PCR, DNA arrays, magnetic capture-hybridization (MCH)-PCR and restriction fragment length polymorphism (RFLP), would contribute to our understanding about different mycotoxigenic fungi detection approaches and will enhance our capability about mycotoxigenic fungi identification, isolation and characterization at critical control points across food and feed chains. We have assessed the principles, results, the limit of detection, and application of these PCR-based detection technologies to alleviate mycotoxins contamination problem in complex food and feed substrates. The potential application of these detection technologies can reduce mycotoxins in complex food and feed matrices. |
| Databáze: | OpenAIRE |
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