O-dealkylation: A newly discovered class of reactions catalysed by the soluble mono-oxygenase of the methanotroph Methylosinus trichosporium 0B3b

Autor:	I. J. Higgins, B. Kaye, S. G. Jezequel
Rok vydání:	1984
Předmět:	chemistry.chemical_classification Methanotroph Stereochemistry Bioengineering General Medicine Alkylation Anisole Applied Microbiology and Biotechnology Catalysis chemistry.chemical_compound Enzyme chemistry Biotransformation Organic chemistry Methanol Biotechnology Demethylation
Zdroj:	Biotechnology Letters. 6:567-570
ISSN:	1573-6776 0141-5492
DOI:	10.1007/bf00135683
Popis:	Cell-free extracts of Methylosinus trichosporium 0B3b (MT 0B3b) containing the soluble, broad specificity methane mono-oxygenase (MMO) have been shown to catalyse yet another type of reaction : O-dealkylation. Several 4-substituted anisoles were investigated as substrates, all showed O-demethylation to varying extents by cell-free extracts of the bacterium. This catalytic ability is common to organisms grown on either methane or methanol as sole carbon source, although the rates of biotransformation are lower for the latter. O-demethylation of anisole itself was inhibited (> 99%) by ethyne, a known MMO inhibitor, strongly indicating that the MMO is the enzyme responsible for this catalysis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fed707ae6e85b214cbfe6881cb8cfbdd https://doi.org/10.1007/bf00135683 Zobrazit plný text záznamu Full text from SpringerLink