Effect of a lotion containing the heat-treated probiotic strain Lactobacillus johnsonii NCC 533 on Staphylococcus aureus colonization in atopic dermatitis
| Autor: | Paul O. Verhoeven, Cyrille H. Haddar, Annick Mercenier, Philippe Andres, V. Bourdès, S. Blanchet‐Réthoré |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Dermatology medicine.disease_cause Gastroenterology Microbiology law.invention 030207 dermatology & venereal diseases 03 medical and health sciences Probiotic 0302 clinical medicine law Internal medicine medicine Colonization SCORAD Lactobacillus johnsonii integumentary system medicine.diagnostic_test biology business.industry Atopic dermatitis biology.organism_classification medicine.disease 030104 developmental biology Real-time polymerase chain reaction Staphylococcus aureus Lotion business |
| Zdroj: | Clinical, Cosmetic and Investigational Dermatology. 10:249-257 |
| ISSN: | 1178-7015 |
| DOI: | 10.2147/ccid.s135529 |
| Popis: | Objective Staphylococcus aureus dominates the skin microbiota in patients with atopic dermatitis (AD), with bacterial loads correlating with disease severity. The aim of this exploratory study was to investigate the effect of a cosmetic lotion containing heat-treated Lactobacillus johnsonii NCC 533 (HT La1) on S. aureus colonization in AD patients. Methods This open-label, multicenter study was performed in AD patients in Germany. First, detection of S. aureus was performed in all patients using the swab or scrub-wash method of sampling, followed by quantitative culture or quantitative polymerase chain reaction. Repeatability and reproducibility of all method combinations were evaluated to select the best combination of sampling and quantification. Second, a lotion containing HT La1 was applied to lesional skin twice daily for 3 weeks. Scoring using local objective SCORing Atopic Dermatitis (SCORAD), measurement of S. aureus load, and lesional microbiome analysis were performed before and after the 3-week treatment period. Results Thirty-one patients with AD were included in the study. All sampling and quantification methods were found to be robust, reproducible, and repeatable for assessing S. aureus load. For simplicity, a combination of swab and quantitative polymerase chain reaction was chosen to assess the efficacy of HT La1. Following application of a lotion containing HT La1 to AD lesions for 3 weeks, a reduction in S. aureus load was observed in patients, which correlated with a decrease in local objective SCORAD. Interestingly, high baseline skin concentrations of S. aureus were associated with good responses to the lotion. Conclusion This study demonstrated that the application of a lotion containing HT La1 to the lesional skin of patients with AD for 3 weeks controlled S. aureus colonization and was associated with local clinical improvement (SCORAD). These findings support further development of topical treatments containing heat-treated nonreplicating beneficial bacteria for patients with AD. |
| Databáze: | OpenAIRE |
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