Colletotrichum siamense as a myco-biocontrol agent for management of the tridax daisy (Tridax procumbens)

Autor: Chettida Srisuksam, Wilawan Wattananukit, Kewarin Klamchao, Boonsri Jongsareejit, Nuchnudda Wichienchote, Alongkorn Amnuaykanjanasin, Piyapat Tepboonrueng, Prawit Yodpanan
Rok vydání: 2020
Předmět:
Zdroj: Physiological and Molecular Plant Pathology. 112:101563
ISSN: 0885-5765
DOI: 10.1016/j.pmpp.2020.101563
Popis: The tridax daisy (Tridax procumbens) is a predominant weed in Thai plantations. Management of weeds in the country, both past and present, heavily relies on the use of synthetic herbicides. Here, we demonstrate the use of a phytopathogen for controlling this weed. The fungus Colletotrichum siamense isolate Cs1 was isolated from a diseased false daisy (Eclipta prostrata). Topical application of a conidial suspension of this fungus on Tridax procumbens plants led to 78–100% disease incidence on days 14–18 post-inoculation. Similar testing in the laboratory and greenhouse settings showed that C. siamense isolate Cs1 was non-pathogenic to cassava (Manihot esculenta) and eight other crops. Fluorescence microscopy of green fluorescent protein-expressing C. siamense indicated that the conidia germinated and formed appressoria for penetration on days 1–7 post-inoculation. Scanning electron microscopy showed hyphae growing on the leaf surface as well as invading the host through stomata. Trichomes of weed leaves served as important infection sites for this myco-pathogen. Oval conidia and pseudohyphae were detected inside the leaf trichomes, indicating that these cell types could help spread the fungus throughout the weed. C. siamense sporulated at high rates, at 1.7 × 1010 conidia/g, on a rice-oat mixture, suggesting a potential production recipe for commercialization. These data suggest that this weed-pathogenic fungus has potential as a bioherbicide for biological control of this weed.
Databáze: OpenAIRE
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