Determination of asymmetric and symmetric dimethylarginines in human plasma by HPLC with electrochemical detection
| Autor: | Josef Tomandl, Zdenek Glatz, Martin Klepárník, Marie Tomandlová |
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| Rok vydání: | 2013 |
| Předmět: |
Analyte
Chromatography Elution 010401 analytical chemistry Extraction (chemistry) Filtration and Separation Electrochemical detection 030204 cardiovascular system & hematology 01 natural sciences High-performance liquid chromatography 0104 chemical sciences Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry Human plasma Reagent Acetonitrile |
| Zdroj: | Journal of Separation Science. 36:3696-3701 |
| ISSN: | 1615-9306 |
| Popis: | A new HPLC method was developed and validated for the determination of asymmetric and symmetric dimethylarginines and l-arginine in human plasma. After SPE and evaporation of the eluate, the samples were derivatised with an o-phthaldialdehyde reagent containing 3-mercaptopropionic acid. The derivatives formed were analysed by isocratic RP-HPLC with electrochemical detection at +320 mV. The mobile phase consisted of 50 mM phosphate buffer (pH 6.1) containing 10% v/v acetonitrile, the flow rate was 1 mL/min. The retention times of all compounds including monomethylarginine (internal standard) were |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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