Infrared Nanoscopy of Alive Biological Cell Surfaces
| Autor: | Fritz Keilmann, Alexandra Goetz, Yasin C. Durmaz |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Terahertz radiation Infrared Graphene Scattering 010401 analytical chemistry 020206 networking & telecommunications Nanotechnology 02 engineering and technology 01 natural sciences 0104 chemical sciences law.invention law Microscopy 0202 electrical engineering electronic engineering information engineering Fluorescence microscope Electron microscope Spectroscopy |
| Zdroj: | 2019 44th International Conference on Infrared, Millimeter, and Terahertz Waves (IRMMW-THz). |
| Popis: | We report progress towards sealing alive cells in their native, wet environment by single-layer graphene, where graphene stabilizes the cell by tightly conforming with its surface and thus enables topographic AFM maps of the cell’s surface. Furthermore, graphene’s transparency to infrared near-fields enables local chemical recognition, at nominally 20-nm spatial resolution, by scattering scanning mid-infrared microscopy (s-SNOM) and spectroscopy (nano-FTIR). Here we demonstrate first infrared nanoscopy results of the surface of native E. Coli cells that show spatial and spectral contrasts. Our study opens a new door into characterizing subcellular structures and components of cells and of fresh tissue sections. Infrared nanoscopy is certainly free of any need for labelling as in super-resolution fluorescence microscopy, and free of fixation and metallization procedures as in electron microscopy that kill and distort cells. |
| Databáze: | OpenAIRE |
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