Production of γ-aminobutyric acid from monosodium glutamate using Escherichia coli whole-cell biocatalysis with glutamate decarboxylase from Lactobacillus brevis KCTC 3498

Autor: Sun Mi Lee, Yung-Hun Yang, Ranjit Gurav, Ye-Lim Park, Hyun-Joong Kim, Hun-Suk Song, Hye Soo Lee, Shashi Kant Bhatia, Jun Young Park, Tae-Rim Choi, Sol Lee Park, Yeong-Hoon Han
Rok vydání: 2020
Předmět:
Zdroj: Korean Journal of Chemical Engineering. 37:2225-2231
ISSN: 1975-7220
0256-1115
DOI: 10.1007/s11814-020-0633-z
Popis: γ-Aminobutyric acid (GABA), an important fine chemical in pharmacotherapy and food industries, is used as a novel material in the nylon industry and has attracted attention for its potential application in large scale production. Search for new genes and strains, development of efficient reaction systems, such as fermentation and bioconversion, and use of cheap starting material like monosodium glutamate (MSG) can make GABA production using less expensive bulk chemicals possible. Therefore, in this study, we constructed a recombinant Escherichia coli whole-cell system for GABA production that expressed glutamate decarboxylase (GAD) from Lactobacillus brevis and used MSG as the starting material. We also optimized the reaction conditions for MSG to GABA conversion, such as citrate buffer concentration, pyridoxal 5′te concentration, temperature, MSG concentration, and cell density (OD600). The optimized whole-cell system converted MSG to GABA via seven repetitive cycles resulting in an average conversion rate of 86% (71.7 mM/h) within 42 h.
Databáze: OpenAIRE
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