Optimization of the antibacterial activity of silver nanoparticles-decorated graphene oxide nanocomposites
| Autor: | Tran Hoang Quan, Phung Ngoc Bao Long, Mai Thanh Phong, Nguyen Huu Hieu, Do Minh Nguyet, Nguyen Thanh Huong, Trinh Ngoc Minh Anh, Doan Ba Thinh, Hoang Minh Nam, Nguyen Minh Dat |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
02 engineering and technology 010402 general chemistry 01 natural sciences Silver nanoparticle law.invention symbols.namesake X-ray photoelectron spectroscopy law Materials Chemistry Fourier transform infrared spectroscopy Antibacterial agent Nanocomposite Graphene Mechanical Engineering Metals and Alloys 021001 nanoscience & nanotechnology Condensed Matter Physics 0104 chemical sciences Electronic Optical and Magnetic Materials Mechanics of Materials symbols 0210 nano-technology Antibacterial activity Raman spectroscopy Nuclear chemistry |
| Zdroj: | Synthetic Metals. 268:116492 |
| ISSN: | 0379-6779 |
| DOI: | 10.1016/j.synthmet.2020.116492 |
| Popis: | In this study, silver/graphene oxide (Ag/GO) nanocomposites were synthesized using the in situ method. Glucose was selected as an eco-friendly reducing agent for the reduction of Ag+ into silver nanoparticles (AgNPs). The characterization of GO and Ag/GO with Fourier transform infrared spectroscopy, transmission electron microscopy, X-ray diffraction, Raman spectroscopy, scanning electron microscopye, energy-dispersive X-ray spectroscopy, and X-ray photoelectron spectroscopy. Results showed that Ag/GO was successfully synthesized. Silver nanoparticles (AgNPs) with an average size of 17.68 ± 4.48 nm were uniformly distributed onto GO sheets. The antibacterial activity of GO, AgNPs, and Ag/GO were tested against Staphylococcus aureus ATCC 25,923 (S. aureus) and Salmonella enterica ATCC 35,664 (S. enterica) bacteria by optical density and plate colony–counting methods. Test results showed that Ag/GO had higher antibacterial activity than its precursors (bare AgNPs and GO) with a bactericidal rate of more than 99.90 %. Thus, Ag/GO was selected for further experimentation. Simultaneous effects of the interaction time, Ag/GO concentration, pH, annealing temperature, and S. aureus density on the antibacterial activity of Ag/GO were also investigated with full factorial experimental design according to Placket–Burman and Box–Behnken designs. After 10 min of interaction time at 37 °C, the optimal values of these factors were determined to be: Ag/GO concentration of 48 μg/mL, pH 5, and S. aureus density 5.3 × 106 CFU/mL, corresponding to an antibacterial capacity of 99.95 % towards S. aureus. The presented experiment confirmed Ag/GO to be able of eliminating about 99.99 % of S. aureus. All results of the present study affirmed the potential applications of Ag/GO as an antibacterial agent in the medical field. |
| Databáze: | OpenAIRE |
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