l-Arginine and nitric oxide synthesis in the cells with inducible NO synthase

Autor:	O. L. Belaia, Z. V. Kuropteva, T. N. Bogatyrenko, L. G. Nagler, L. M. Baider
Rok vydání:	2019
Předmět:	chemistry.chemical_classification Nitric oxide synthesis Arginine 010405 organic chemistry Oxide General Chemistry 010402 general chemistry 01 natural sciences Molecular biology 0104 chemical sciences chemistry.chemical_compound Enzyme chemistry Citrulline Ammonium chloride Incubation Ex vivo
Zdroj:	Russian Chemical Bulletin. 68:174-180
ISSN:	1573-9171 1066-5285
Popis:	The effect of citrulline and ammonium chloride on the nitric oxide formation by peritoneal macrophages and liver tissue cells was studied using ESR spectroscopy. In ex vivo models, the incubation of cells capable of expressing inducible NO synthase (iNOS) with interferon-γ resulted in a moderate increase in the amount of hemoglobin–nitric oxide nitrosyl complexes (Heme–NO NCs), whereas incubation with l-citrulline and ammonium chloride increased the amount of Heme–NO NCs by an order of magnitude. It was assumed that a separate cycle of L-arginine and nitric oxide synthesis exists in the peritoneal macrophages and liver cells, with the major participants of the cycle being the inducible NO synthase enzyme (iNOS) and enzymes that synthesize L-arginine from L-citrulline and a nitrogen source. Functioning of this cycle makes immunocompetent cells with iNOS able to produce NO for a long time and in large amounts.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fe807be9ff32281223b9171ab72b114b https://doi.org/10.1007/s11172-019-2434-2 Zobrazit plný text záznamu Full text from SpringerLink