Stereoselective synthesis of L-[15N] amino acids with glucose dehydrogenase and galactose mutarotase as NADH regenerating system
| Autor: | N. Bucurenci, N. Palibroda, Iulia Lupan, Octavian Popescu, Maria Chiriac |
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| Rok vydání: | 2008 |
| Předmět: |
chemistry.chemical_classification
education.field_of_study Stereochemistry Organic Chemistry Isomerase Leucine dehydrogenase Biochemistry Analytical Chemistry Amino acid Enzyme Stereospecificity chemistry Glucose dehydrogenase Drug Discovery Radiology Nuclear Medicine and imaging Stereoselectivity Galactose mutarotase education Spectroscopy |
| Zdroj: | Journal of Labelled Compounds and Radiopharmaceuticals. 51:171-174 |
| ISSN: | 1099-1344 0362-4803 |
| DOI: | 10.1002/jlcr.1496 |
| Popis: | We have developed an efficient stereospecific enzymatic synthesis of L-[15N]-valine, L-[15N]-leucine, L-[15N]-norvaline, L-[15N]-norleucine and L-[15N]-isoleucine from the corresponding α-keto acids by coupling the reactions catalysed by leucine dehydrogenase and glucose dehydrogenase/galactose mutarotase. Giving high yields of L-amino acids, the procedure is economical and easy to perform and to monitor at a synthetically useful scale (1–10 g). Copyright © 2008 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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