Modified and improved anti-acetylcholine receptor (AChR) antibody assay: comparison of analytical and clinical performance with conventional anti-AChR antibody assay
| Autor: | Luca Durelli, Roberto Pagni, Bruno Bergamasco, L. Bergamini, Giuseppe Aimo, M. R. Bongioanni, B. Ferrero |
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| Rok vydání: | 1997 |
| Předmět: |
medicine.medical_specialty
Biochemistry (medical) Clinical Biochemistry Curve analysis Clinical performance Biology medicine.disease Control subjects Molecular biology Myasthenia gravis Endocrinology Internal medicine medicine biology.protein Antibody Receptor Quantitative analysis (chemistry) Acetylcholine receptor |
| Zdroj: | Clinical Chemistry. 43:824-831 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/43.5.824 |
| Popis: | We developed a modified anti-acetylcholine receptor (AChR) antibody (Ab) assay based on a radioreceptor assay and a calibration curve. We compared the analytical and clinical performances of this modified assay with those of the conventional anti-AChR Ab radioreceptor assay. Serum specimens were from patients with myasthenia gravis (MG) (n = 156) and from control subjects (n = 106). The modified assay demonstrated lower within-assay (4.0–6.6%) and between-assay (5.3–7.8%) CVs, greater linearity, lower cost, and shorter assay time than the conventional method. ROC curve analysis indicated almost identical specificity and sensitivity (>0.92) for these two anti-AChR Ab assays. The modified and conventional assays were also equivalent for blocking anti-AChR Ab assay. Moreover, the modified anti-AChR Ab assay, differently from the conventional assay, allowed us to reveal anti-AChR Ab concentration differences among different clinical grades of MG. |
| Databáze: | OpenAIRE |
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