Isolation and ex vivo expansion of murine bone marrow-derived group 2 innate lymphoid cells (MUC8P.734)
| Autor: | Claudia Duerr, Connor McCarthy, Barbara Mindt, Jorg Fritz |
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| Rok vydání: | 2015 |
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| Zdroj: | The Journal of Immunology. 194:204.14-204.14 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Group 2 innate lymphoid cells (ILC2) are recently discovered lymphocyte subpopulations that have innate ability to produce large quantities of a specific set of cytokines regulating innate and adaptive immune responses. Importantly, deregulated ILC2 responses are implicated in detrimental type 2 immunopathologies such as allergies, asthma and atopic dermatitis, as well as fibrotic disease, both in rodents and humans. However, the progress in our understanding of ILC2 biology has been hampered by their scarcity at steady state as well as infected and inflamed tissues. Here, a simple, efficient and reliable method is presented to isolate murine ILC2 from the bone marrow by flow cytometric cell sorting and further cultivate them using a defined cytokine cocktail, yielding a 500-fold expansion. The resulting ILC2 constitute a pure cell population and show the same phenotypic and functional characteristics as ILC2 in vivo. Thus, this protocol enables a high yield of ILC2 from murine bone marrow and thereby provides sufficient ILC2 numbers for standard cell and molecular biology assays. An in-depth understanding of the cellular and molecular mechanisms of ILC2 is essential for the development of new strategies and drugs to fight unwanted type 2 immunopathologies and fibrotic disease. |
| Databáze: | OpenAIRE |
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