Molecular Characterization of Monochloroacetate-DegradingArthrobactersp. Strain D2 Isolated from Universiti Teknologi Malaysia Agricultural Area

Autor: Fahrul Huyop, Robert J. Lamis, Tengku Haziyamin Tengku Abdul Hamid, Ronnie G. Gicana, Azzmer Azzar Abdul Hamid, Duha Alomar, Elham Khosrowabadi
Rok vydání: 2014
Předmět:
Zdroj: Bioremediation Journal. 18:12-19
ISSN: 1547-6529
1088-9868
DOI: 10.1080/10889868.2013.834867
Popis: Arthrobacter sp. strains D2 and D3 and Labrys sp. strain D1 capable of degrading 20 mM monochloroacetic acid (MCA) were isolated from soil contaminated with herbicides and pesticides. All three isolates were able to grow on MCA as the sole source of carbon and energy with concomitant chloride ion release in the growth medium (19 mM). Strains D2 and D3 (cells doubling time 7 ± 0.3 h) grew four times faster than D1 (26 ± 0.1 h). Strain D2 was then further investigated and could also grow in 10 mM of monobromoacetic acid (MBA), 2,2-dichloropropionic acid (2,2DCP), d,l-2-chloropropionic acid (D,L2CP), l-2-chloropropionic acid (L-2CP), d-2-chloropropionic acid (D-2CP), and glycolate as the sole sources of carbon and energy. Dehalogenase gene amplification using group I primers revealed a 410-bp polymerase chain reaction (PCR) product, but there was none using group II primers. The partial amino acid sequence analysis of group I DehD2 dehalogenase showed at least 32% identity to the corresponding regio...
Databáze: OpenAIRE
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