Selective immobilization of bacterial light-harvesting proteins and their photoelectric responses
| Autor: | Kenji V. P. Nagashima, Shunsuke Yajima, Takehisa Dewa, Masaharu Kondo, Kouji Iida, Morio Nagata, Kaori Harada, Rei Furukawa, Mamoru Nango |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Photocurrent Materials science 030102 biochemistry & molecular biology Absorption spectroscopy biology Substrate (chemistry) Photochemistry biology.organism_classification Fluorescence 03 medical and health sciences Rhodobacter sphaeroides 030104 developmental biology Membrane Electrode General Materials Science Photosynthetic membrane |
| Zdroj: | MRS Communications. 8:1124-1128 |
| ISSN: | 2159-6867 2159-6859 |
| DOI: | 10.1557/mrc.2018.162 |
| Popis: | With the aim of understanding the excitation energy transfer mechanism in natural photosynthetic membranes, light-harvesting (LH)2 and LH1-reaction center, which are pigment-protein complexes separated from Rhodobacter sphaeroides, were aligned on a planar electrode surface in stripe patterns at 5 urn intervals. Observation of the absorption spectrum and fluorescence microphotographs revealed selective immobilization and conservation of the pigments. Photocurrent signals were obtained when the electrode was illuminated at either 880 or 800 nm. The fabricated structure was confirmed to function as a natural photosynthetic membrane with the highest photocurrent signal being obtained when using a co-immobilized substrate under excitation at 800 nm. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink