Molecular Description of Macroorchis spinulosus (Digenea: Nanophyetidae) Based on ITS1 Sequences
| Autor: | Dong Wook Ryang, Bong-Kwang Jung, Deok-Gyu Kim, Eun Jeong Won, Jong-Yil Chai, Yong Woon Yun, Jaeeun Cho, Min Jae Kim |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Phylogenetic tree Intermediate host Zoology Anatomy 030108 mycology & parasitology Ribosomal RNA Biology biology.organism_classification 18S ribosomal RNA Digenea 03 medical and health sciences 030104 developmental biology Infectious Diseases Paragonimus parasitic diseases Sucker Parasitology Trematoda |
| Zdroj: | The Korean Journal of Parasitology. 54:109-112 |
| ISSN: | 1738-0006 0023-4001 |
| DOI: | 10.3347/kjp.2016.54.1.109 |
| Popis: | We performed a molecular genetic study on the sequences of 18S ribosomal RNA (ITS1 region) gene in 4-day-old adult worms of Macroorchis spinulosus recovered in mice experimentally infected with metacercariae from crayfish in Jeollanam-do Province, Korea. The metacercariae were round, 180 μm in average diameter, encysted with 2 layers of thick walls, but the stylet on the oral sucker was not clearly seen. The adult flukes were oval shape, and 760-820 μm long and 320-450 μm wide, with anterolateral location of 2 large testes. The phylogenetic tree based on ITS1 sequences of 6 M. spinulosus samples showed their distinguished position from other trematode species in GenBank. The most closely resembled group was Paragonimus spp. which also take crayfish or crabs as the second intermediate host. The present study is the first molecular characterization of M. spinulosus and provided a basis for further phylogenetic studies to compare with other trematode fauna in Korea. |
| Databáze: | OpenAIRE |
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