A study of the antioxidants in Oxytropis pilosa (L.) DC

Autor:	Stevo Najman, Ljiljana C. Miladinović, Dragoljub L. Miladinović
Rok vydání:	2011
Předmět:	chemistry.chemical_classification Antioxidant biology Superoxide medicine.medical_treatment General Chemistry Glutathione Superoxide dismutase chemistry.chemical_compound Oxytropis pilosa chemistry Biochemistry Catalase biology.protein medicine Carotenoid Peroxidase
Zdroj:	Journal of the Serbian Chemical Society. 76:505-512
ISSN:	1820-7421 0352-5139
DOI:	10.2298/jsc100701045m
Popis:	The objective of this study was to estimate the antioxidative potential of Oxytropis pilosa (L.) DC. during the active vegetative period. The activities of the antioxidant enzymes (superoxide dismutase, catalase, peroxidase), the quantities of malonyldialdehyde, superoxide and hydroxyl radicals and reduced glutathione and the content of total flavonoids, chlorophylls a and b, caro- tenoids and soluble proteins were determined. The results showed that extracts from all plant organs exhibited antioxidant activity. The highest antioxidant ability was observed in the leaves where all the investigated antioxidant en- zymes were active in a specific way: During the spring season, peroxidase showed the maximum activity 18.54 U mg -1 protein, catalase peaked in sum- mer 9.04 U mg -1 protein, whereas, during the autumn season, superoxide dis- mutase showed maximum activity, 54.28 U mg -1 protein. Reduced glutathione, pigments and carotenoids present in the leaves contribute to the high antioxi- dant activity. Furthermore, inhibition of chemiluminescence activity of Balb/c mice blood phagocytes by crude leaf extracts at concentrations of 3.5 and 7.0 μg cm -3 were 30.2 and 36.5 %, respectively.
Databáze:	OpenAIRE
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