Autor: | Ursula Müller-Werdan, Karl Werdan, Doris Jäger, Jürgen Holtz, Sylvana P. Müller, Klaus Pönicke |
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Rok vydání: | 2002 |
Předmět: |
medicine.medical_specialty
Clinical chemistry medicine.medical_treatment Clinical Biochemistry Hemodynamics Cell Biology General Medicine Biology Muscle hypertrophy Elongation factor Insulin-like growth factor Endocrinology Apoptosis Internal medicine ADP-ribosylation medicine Pseudomonas exotoxin Molecular Biology |
Zdroj: | Molecular and Cellular Biochemistry. 229:25-34 |
ISSN: | 0300-8177 |
DOI: | 10.1023/a:1017982724938 |
Popis: | Because of its key role in proteosynthesis, the total content of elongation factor-2 (EF-2) and the distribution of six main EF-2 variants were investigated after Pseudomonas Exotoxin A catalyzed [32P]ADP-ribosylation using 1D-PAGE and isoelectric focusing (IEF) in a rat model of hemodynamic overload with variable degrees of cardiac hypertrophy: Chronic NO-synthase inhibition by L-NAME (N-omega-nitro-L-arginine-methyl-esther; 0.75 mg/ml drinking water) induced arterial hypertension without hypertrophy but myocardial apoptosis; additional treatment with IGF-1 (osmotic micropumps) did not modify hypertension but reduced apoptosis allowing moderate hypertrophy of the left ventricles. Total EF-2 did not significantly increase in rats with hemodynamic overload with or without IGF-1 supplementation. A positive correlation was found between an acidic EF-2 variant and apoptosis (p = 0.01). Hypertrophy under additional IGF-1 was combined with a shift of the EF-2 variants to basic subtypes (p < 0.01). This finding may be indicative of the trophic potency of IGF-1. |
Databáze: | OpenAIRE |
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