Regulation of the Sox3 Gene in an X0/X0 Mammal without Sry, the Amami Spiny Rat, Tokudaia osimensis
| Autor: | Shusei Mizushima, Takamichi Jogahara, Kohei Washio, Asato Kuroiwa |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Reporter gene biology Tokudaia osimensis Y chromosome biology.organism_classification Molecular biology Tokudaia tokunoshimensis 03 medical and health sciences 0302 clinical medicine Testis determining factor Genetics Tokudaia Enhancer Molecular Biology 030217 neurology & neurosurgery Genetics (clinical) X chromosome 030304 developmental biology |
| Zdroj: | Cytogenetic and Genome Research. 159:143-150 |
| ISSN: | 1424-859X 1424-8581 |
| Popis: | Two species of spiny rats, Tokudaia osimensis and Tokudaia tokunoshimensis, show an X0/X0 sex chromosome constitution due to the lack of a Y chromosome. The Sry gene has been completely lost from the genome of these species. We hypothesized that Sox3, which is thought to be originally a homologue of Sry, could function in sex determination in these animals in the absence of Sry. Sox3 was localized in a region of the X chromosome in T. osimensis homologous to mouse. A similar testis- and ovary-specific pattern of expression was observed in mouse and T. osimensis. Although the sequence of the Sox3 gene and its promoter are highly conserved, a 13-bp deletion was specifically found in the promoter region of the 2 spiny rat species. Reporter gene assays were performed to examine the effect of the 13-bp deletion in the promoter region on Sox3 regulation. Although an approximately 60% decrease in activity was observed using the Tokudaia promoters with the 13-bp deletion, the activity was recovered using a mutated promoter in which the deletion was filled with mouse sequence. To evaluate whether SOX3 could regulate Sox9 expression, a reporter gene assay was carried out using testis-specific enhancer of Sox9 core (TESCO). Co-transfection with a combination of mouse SF1 and mouse SOX3 or T. osimensis SOX3 resulted in a greater than 2-fold increase in activity of mouse and T. osimensis TESCO. These results support the idea that the function of SOX3 as a transcription factor, as has been reported in mice and humans, is conserved in T. osimensis. Therefore, we conclude that the Sox3 gene has no function in sex determination in Sry-lacking Tokudaia species. |
| Databáze: | OpenAIRE |
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