The relationship between oxygen and adenosine in astrocytic cultures
| Autor: | Tobias B Kulik, German Echeverry, H. Richard Winn, Shimon N. Aronhime, Alex Beylin |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Adenosine monophosphate
medicine.medical_specialty Biology Nucleoside transporter Adenosine Oxygen tension Cellular and Molecular Neuroscience chemistry.chemical_compound Endocrinology Neurology chemistry Adenine nucleotide Internal medicine Extracellular medicine biology.protein Inosine Nucleoside medicine.drug |
| Zdroj: | Glia. 58:1335-1344 |
| ISSN: | 0894-1491 |
| DOI: | 10.1002/glia.21011 |
| Popis: | Brain tissue oxygenation affects cerebral function and blood flow (CBF). Adenosine (Ado), a purine nucleoside, moderates neuronal activity, and arterial diameter. The cellular source of Ado in brain remains elusive; however, astrocytes are a logical site of production. Using astrocytic cultures, we tested the hypothesis that astrocytic derived Ado reflects cerebral oxygenation. We found that during alterations in pO(2), extracellular levels of Ado [Ado](e) changed rapidly. Graded reductions of oxygen tension revealed that[Ado](e) reached 10(-7) M to 10(-6) M with a pO(2) of 30-10mmHg, comparable with [Ado](e) and oxygen levels found in brain tissue during normoxemia. Higher O(2) levels were associated with a depression of [Ado](e). Under conditions of low pO(2) (pO(2) |
| Databáze: | OpenAIRE |
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