Colon cancer-associated transcript-1 enhances glucose metabolism and colon cancer cell activity in a high-glucose environment in vitro and in vivo
| Autor: | Yan Luo, Yifan Luo, Hongchang Zhou, Feng Wenming, Yandi Sun, Hui Gong, Ge Cui, Yuxuan Huang, Ting Zhang, Mengya Zhang, Yunliang Yao, Jun Liu, Xi-Ning Li |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty business.industry Insulin medicine.medical_treatment Gastroenterology Metabolism Carbohydrate metabolism Lactic acid 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Endocrinology Oncology chemistry Apoptosis 030220 oncology & carcinogenesis Internal medicine Medicine Glycolysis business Protein kinase B PI3K/AKT/mTOR pathway |
| Zdroj: | Journal of Gastrointestinal Oncology. 11:1164-1185 |
| ISSN: | 2219-679X 2078-6891 |
| Popis: | Background Our study aims to investigate the effect of colon cancer-associated transcript-1 (CCAT-1) on colon cancer cells' activity and metabolism under different glucose environments in vitro and in vivo. Methods The levels of proliferation, migration, glucose, lactic acid, glucose metabolism-related enzymes, apoptosis genes, epithelial-mesenchymal transition (EMT) marker proteins, and PI3K/Akt/C-MYC pathway in CCAT-1-silenced SW620 cells cultured with different glucose levels were tested. Twenty BALB/C nude mice with hyperglycemia or normal blood sugar were transplanted with CCAT-1-silenced SW620 cells, blood glucose levels, lactic acid, insulin, and volume of transplanted tumor cells, the expression of EMT marker proteins, and PI3K/Akt/C-MYC pathway was detected. Results The levels of proliferation, migration, glucose, lactic acid, LDH-A, PKM2, and HK2 decreased, apoptosis increased in SW620 cells cultured with low glucose or silenced CCAT-1 (P |
| Databáze: | OpenAIRE |
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