The Role of Chitinase Gene Expression in the Defense of Harvested Banana Against Anthracnose Disease

Autor:	Wanli Tang, Ling-Ling Li, Donald E. Irving, Lu-Bin Zhang, Shijiang Zhu, Chuxiong Zhuang, Bao-Cheng Ma, Li-Yan Ma
Rok vydání:	2009
Předmět:	Methyl jasmonate biology food and beverages Ripening Horticulture Colletotrichum musae Plant disease resistance Pathogenic fungus biology.organism_classification Microbiology chemistry.chemical_compound chemistry Biochemistry Gene expression Chitinase Genetics biology.protein Gene
Zdroj:	Journal of the American Society for Horticultural Science. 134:379-386
ISSN:	2327-9788 0003-1062
Popis:	The pathogenic fungus Colletotrichum musae infects developing green bananas (Musa spp. AAA group), but remains latent until the fruit ripens. The aim of this research was to determine whether the appearance of disease symptoms is regulated by chitinase gene expression following treatment of fruit with benzothiadiazole (BTH) and methyl jasmonate (MeJA), and with physical (heat) and chemical (H2O2 and Ca2+-related) treatments. In bananas inoculated with C. musae, BTH and MeJA lowered disease severity and stimulated higher gene expression compared with the untreated controls during ripening. However, in naturally infected bananas, BTH and MeJA treatments slightly reduced transcription of the chitinase gene in green bananas, but they prolonged gene expression in ripe bananas and significantly reduced disease severity. The combination of H2O2 and the NADPH oxidase inhibitor, diphenylene iodonium, down-regulated chitinase gene expression and compromised disease resistance compared with H2O2 alone. Heat treatment (HT) or the combination of HT followed by CaCl2 reduced disease, but only the latter significantly upregulated chitinase gene expression. The combination of HT and a calcium ionophore (A23187) resulted in different disease indicies and different levels of gene expression depending upon the order of application: HT followed by A23187 induced higher gene expression and lower disease. The results suggest that disease resistance of green bananas could be related to high and prolonged levels of chitinase gene expression, and chitinase could be involved in harvested banana's anthracnose resistance activated by different defense pathway signals, such as BTH, MeJA, H2O2, and Ca2+.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fe1def0d2ce6e461281a4e4a6fbb06bf https://doi.org/10.21273/jashs.134.3.379 Zobrazit plný text záznamu