In silico and in vivo protective effect of Morus nigra leaves on oxidative damage induced by iron overload
| Autor: | Roberto Marinho Maciel, Ana Martiele Engelmann, Kássia Caroline Figueredo, Thiago Duarte, Cristiane Cademartori Danesi, Liliane de Freitas Bauermann, Cibele Lima Lhamas, Gilberti Helena Hübscher Lopes, Camille Gaube Guex, Marta M.M.F. Duarte, Andreia Regina Haas da Silva |
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| Rok vydání: | 2021 |
| Předmět: |
Pharmacology
Chemical Health and Safety biology medicine.diagnostic_test Chemistry Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health General Medicine Toxicology medicine.disease biology.organism_classification medicine.disease_cause Ferulic acid chemistry.chemical_compound Hepcidin medicine Caffeic acid biology.protein Serum iron Kaempferol Morus nigra Hemochromatosis Oxidative stress |
| Zdroj: | Drug and Chemical Toxicology. 45:2814-2824 |
| ISSN: | 1525-6014 0148-0545 |
| Popis: | Morus nigra L. is a plant popularly known as 'amoreira preta', very used in folk medicine. Iron overload (hemochromatosis) is a clinical condition that causes damage to various tissues due to oxidative stress. Therapy to control iron overload is still unsatisfactory. The protective effect on oxidative stress induced by iron overload was verified. Phytochemical characterization was evaluated by UHPLC-MS/MS. The in silico toxicity predictions of the main phytochemicals were performed via computer simulation. To induce iron overload, the animals received iron dextran (50 mg/kg/day). The test groups received doses of 500 and 1000 mg/kg of M. nigra extract for six weeks. Body weight, organosomatic index, serum iron, hepatic markers, cytokines, interfering factors in iron metabolism, enzymatic and histopathological evaluations were analyzed. Vanillic acid, caffeic acid, 6-hydroxycoumarin, p-coumaric acid, ferulic acid, rutin, quercitrin, resveratrol, apigenin and kaempferol were identified in the extract. In addition, in silico toxic predictions showed that the main compounds presented a low probability of toxic risk. The extract of M. nigra showed to control the mediators of inflammation and to reduce iron overload in several tissues. Our findings illustrate a novel therapeutic action of M. nigra leaves on hemochromatosis caused by iron overload. |
| Databáze: | OpenAIRE |
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