Manganese dioxide-core–shell hyperbranched chitosan (MnO2–HBCs) nano-structured screen printed electrode for enzymatic glucose biosensors
| Autor: | Hala S. Abd El-Haleem, Amr Hefnawy, Ashraf H. Badawi, Rabeay Y. A. Hassan, Ibrahim M. El-Sherbiny |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Detection limit
Immobilized enzyme Chemistry General Chemical Engineering Metal ions in aqueous solution Glucose Measurement technology industry and agriculture chemistry.chemical_element macromolecular substances 02 engineering and technology General Chemistry Manganese 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Chitosan Electron transfer chemistry.chemical_compound Organic chemistry 0210 nano-technology Biosensor Nuclear chemistry |
| Zdroj: | RSC Advances. 6:109185-109191 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/c6ra24419j |
| Popis: | In this study, the synthesis, characterization and testing of new polymeric–metal oxide nanocomposites for enzymatic glucose biosensors were performed. Among various nano-composites, manganese dioxide-core–shell hyperbranched chitosan (MnO2–HBCs) provided rapid and high efficiency direct electron transfer from the redox active centre of an immobilized enzyme and screen printed electrode. The assay optimization was achieved after testing the effects of several factors such as type of crosslinking agent, accumulation potential, toxicity of heavy metals and interferences on the bioactivity of GOx. Results demonstrated sensitivity of the proposed method to detect inhibition effects of metal ions and also the response of agents interfering with glucose measurement. A chronoamperometric calibration curve was obtained, and the oxidation current of the enzymatically produced H2O2 was linearly dependent on glucose concentration with a detection limit of 7 μg mL−1. Thus, the clinical determination of glucose concentration was performed on blood samples and the results were correlated with a reference method. In conclusion, the current study suggests a new class of electrochemical biosensors and paves the way for further promising applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|