Primary Structure and Function of Three Gonadotropin-Releasing Hormones, Including a Novel Form, from an Ancient Teleost, Herring1
| Autor: | Joachim Carolsfeld, Minkyu Park, John P. Chang, Jean Rivier, Nancy M. Sherwood, J.F.F. Powell, Wolfgang H. Fischer, A. Grey Craig |
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| Rok vydání: | 2000 |
| Předmět: |
endocrine system
Pituitary gland medicine.medical_specialty Edman degradation medicine.drug_class Protein primary structure Vertebrate Pacific herring Gonadotropin-releasing hormone Biology biology.organism_classification Endocrinology medicine.anatomical_structure Herring Internal medicine biology.animal medicine Gonadotropin hormones hormone substitutes and hormone antagonists |
| Zdroj: | Endocrinology. 141:505-512 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo.141.2.7300 |
| Popis: | The evolution of GnRH and the role of multiple forms within the brain are examined. Three forms of GnRH were purified from the brain of Pacific herring (Clupea harengus pallasi) and characterized using Edman degradation and mass spectrometry. Two forms correspond with the known structures of chicken GnRH-II and salmon GnRH that are found in many vertebrate species. The third form, designated herring GnRH (hrGnRH), has a primary structure of pGlu-His-Trp-Ser-His-Gly-Leu-Ser-Pro-Gly-NH2. This novel peptide is a potent stimulator of gonadotropin II and GH release from dispersed fish pituitary cells. The content of hrGnRH in the pituitary was 8-fold that of salmon GnRH and 43-fold that of chicken GnRH-II, which provides supporting evidence that hrGnRH is involved in the release of gonadotropin. Herring is the most phylogenetically ancient animal in which three forms of GnRH have been isolated and sequenced. Our evidence suggests that the existence of three GnRHs in the brain of one species 1) is an ancestral ... |
| Databáze: | OpenAIRE |
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