IL‐10 promotes malignant pleural effusion in mice by regulating T H 1‐ and T H 17‐cell differentiation and migration

Autor: Xiu-Zhi Wu, Xue-Bin Pei, Yao Wang, Huan-Zhong Shi, Feng-Shuang Yi, Wen Wang, Zhen Wang, Li-Li Xu, Xin-Yu Shi, Kan Zhai
Rok vydání: 2019
Předmět:
Zdroj: European Journal of Immunology. 49:653-665
ISSN: 1521-4141
0014-2980
DOI: 10.1002/eji.201847685
Popis: The role of IL-10 in malignant pleural effusion (MPE) remains unknown. By using murine MPE models, we observed that an increase in pleural IL-10 was a significant predictor of increased risk of death. We noted that TH 1- and TH 17-cell content in MPE was higher in IL-10-/- mice than in WT mice, and IL-10 deficiency promoted differentiation into TH 1 but not into TH 17 cells. A higher fraction of TH 1 and TH 17 cells in the MPE of IL-10-/- mice expressed CXCR3 compared with WT mice. We also demonstrated that Lewis lung cancer and colon adenocarcinoma cells secreted large amounts of CXCL10, a ligand of CXCR3, which induced the migration of TH 1 and TH 17 cells into the MPE, and IFN-γ could promote this signaling cascade. Furthermore, intrapleural injection of mice with CXCL10-deficient tumor cells led to decreased TH 1- and TH 17-cell content in MPE, increased MPE volume, and reduced survival of MPE-bearing mice. Taken together, we demonstrated that IL-10 deficiency promoted T-cell differentiation into TH 1 cells and upregulated the CXCR3-CXCL10 signaling pathway that recruits TH 1 and TH 17 cells into MPE, ultimately resulting in decreased MPE formation and longer survival time of mice-bearing MPE.
Databáze: OpenAIRE
Externí odkaz: