Effects of LB Broth, Naphthalene Concentration, and Acetone on the Naphthalene Degradation Activities by Pseudomonas putida G7
| Autor: | Liu Xuegong, Yao Wan, Honggui Zhang, Liu Bo, Zhang Kai, Chang Suyun, Ren Biqiong |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
biology Ecological Modeling Metabolism biology.organism_classification Pollution Pseudomonas putida Solvent chemistry.chemical_compound Enzyme chemistry Acetone biology.protein Environmental Chemistry Organic chemistry Degradation (geology) Waste Management and Disposal Catechol dioxygenase Water Science and Technology Naphthalene |
| Zdroj: | Water Environment Research. 87:61-67 |
| ISSN: | 1554-7531 1061-4303 |
| DOI: | 10.2175/106143014x14062131179078 |
| Popis: | Luria-Bertani broth and acetone were usually used in naphthalene degradation experiments as nutrient and solvent. However, their effect on the degradation was seldom mentioned. In this work, we investigated the effect of LB, naphthalene concentration, and acetone on the degradation of naphthalene by Pseudomonas putida G7, which is useful for the degradation of naphthalene on future field remediation. By adding LB, the naphthalene degradation efficiencies and naphthalene dioxygenase were both decreased by 98%, while the catechol dioxygenase was decreased by 90%. Degradation of naphthalene was also inhibited when naphthalene concentration was 56 ppm and higher, which was accompanied with the accumulation of orange-colored metabolism products. However, acetone can stimulate the degradation of naphthalene, and the stimulation was more obvious when naphthalene concentration was lower than 2000 ppm. By assaying the enzyme activities of naphthalene dioxygenase and catechol dioxygenase, it was thought that the degradation efficiency was depending on the more sensitive enzymes on the complicated conditions. |
| Databáze: | OpenAIRE |
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