Ovarian Toxicity of 2‐Bromopropane in the Non‐Pregnant Female Rat

Autor: Naomi Hisanaga, Nobuyuki Asaeda, Kei-ichiro Maeda, Yasuhiro Takeuchi, Gaku Ichihara, Hiroko Tsukamura, Michihiro Kamijima, Junzoh Kitoh, Tamie Nakajima, Zhenlin Xie, Xiaozhong Yu
Rok vydání: 1997
Předmět:
Zdroj: Journal of Occupational Health. 39:144-149
ISSN: 1348-9585
1341-9145
DOI: 10.1539/joh.39.144
Popis: Ovarian Toxicity of 2-Bromopropane in the Non-Pregnant Female Rat: Michihiro KAMIJIMA, et al. Department of Hygiene, Nagoya University School of Medicine—A cluster of patients with amenorrhea, oligospermia and anemia were found among workers in an electronics factory in the Republic of Korea. 2- Bromopropane was suspected to cause the disorders. This study aimed to clarify its ovarian toxicity in the virgin rat. Female Wistar rats were daily exposed to 0, 100, 300, or 1,000 ppm 2-bromopropane for eight hours a day for 9 weeks. During the experimental period, vaginal smears were taken everyday to monitor ovarian cyclicity. Tissues were histopathologically examined and plasma concentrations of luteinizing hormone (LH) and follicle- stimulating hormone (FSH) were determined at the end of experiment. The vaginal smear test showed that the number of normal cycles decreased significantly both in the 300 and 1,000 ppm groups. The histopathological examination revealed dose-dependent ovarian follicle atresia accompanied by a decreased number of normal antral and growing follicles in the 300 and 1,000 ppm groups. Especially, in the ovaries of rats with persistent estrous smears in the 1,000 ppm group, most of the follicles were atretic and there were no newly formed corpora lutea. There still remained normal antral follicles and corpora lutea in the ovaries of the remaining rats of the group and of the 300 ppm group with constant diestrous and occasional estrous smears. Hormonal examination revealed no statistically significant change
Databáze: OpenAIRE
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