Dynamin 2 Regulates Granule Exocytosis during NK Cell-Mediated Cytotoxicity

Autor:	Colin M. Segovis, Renee A. Schoon, Paul J. Leibson, Daniel D. Billadeau, Christopher J. Dick, Timothy S. Gomez, Zhenkun Lou, Laura N. Arneson
Rok vydání:	2008
Předmět:	Small interfering RNA Innate immune system Lytic cycle Immunology Granule (cell biology) Immunology and Allergy GTPase Biology Cytotoxicity Exocytosis Cell biology Dynamin
Zdroj:	The Journal of Immunology. 181:6995-7001
ISSN:	1550-6606 0022-1767
DOI:	10.4049/jimmunol.181.10.6995
Popis:	NK cells are innate immune cells that can eliminate their targets through granule release. In this study, we describe a specialized role for the large GTPase Dynamin 2 (Dyn2) in the regulation of these secretory events leading to cell-mediated cytotoxicity. By modulating the expression of Dyn2 using small interfering RNA or by inhibiting its activity using a pharmacological agent, we determined that Dyn2 does not regulate conjugate formation, proximal signaling, or granule polarization. In contrast, during cell-mediated killing, Dyn2 localizes with lytic granules and polarizes to the NK cell–target interface where it regulates the final fusion of lytic granules with the plasma membrane. These findings identify a novel role for Dyn2 in the exocytic events required for effective NK cell-mediated cytotoxicity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fdf2c45ebf5f6db297c78bbdb5e51f7e https://doi.org/10.4049/jimmunol.181.10.6995 Zobrazit plný text záznamu