Monoclonal Antibodies to Porcine Thermal-Stable Muscle Protein for Detection of Pork in Raw and Cooked Meats

Autor:	Y-H.Peggy Hsieh, Fur-Chi Chen, Roger C. Bridgman
Rok vydání:	2008
Předmět:	Muscle protein Chromatography Protein band Anticorps monoclonal Chemistry medicine.drug_class technology industry and agriculture food and beverages Elisa assay Monoclonal antibody Epitope Hybridoma cell Antigen medicine Food Science
Zdroj:	Journal of Food Science. 63:201-205
ISSN:	0022-1147
Popis:	Four hybridoma cell lines secreting monoclonal antibodies (MAbs) specific to porcine thermal-stable muscle proteins (TSMPs) were developed. The MAbs reacted with three protein bands (20.5, 22 and 24 kD) from raw pork extract; the protein band (24 kD) which was also present in cooked pork was identified as porcine-specific TSMP. Epitope analysis indicated four MAbs recognized same or closely located antigenic sites on the pork TSMP. The developed MAb-based indirect enzyme-linked immunosorbent assay (ELISA) enabled the detection of pork in raw and cooked heterogeneous meat mixtures as low as 10 glkg. The curvilinear relations of second-degree polynomial (r 2 > 0.995) between pork concentrations and ELISA responses enabled quantifying degree of adulteration of pork in meat products.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fde95c292da3b31a4ea73c08bcd0e66b https://doi.org/10.1111/j.1365-2621.1998.tb15709.x Zobrazit plný text záznamu Plný text