Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) Using Bacterial Whole Cells as Molecular Probes
| Autor: | Paul F. McKay, Camilla A Gladstone, Fadil A. Bidmos, Yan-Ping Guo, Robin J. Shattock, Paul R. Langford, Sara Siris, Christopher L. Pinder |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.drug_class Neisseria meningitidis Biology medicine.disease_cause Acquired immune system Monoclonal antibody Microbiology law.invention 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Antigen law medicine biology.protein Recombinant DNA Antibody Molecular probe Pathogen 030215 immunology |
| Zdroj: | Vaccine Delivery Technology ISBN: 9781071607947 |
| Popis: | The immunoglobulin capture assay (ICA) enables the enrichment for pathogen-specific plasmablasts from individuals with a confirmed adaptive immune response to vaccination or disseminated infection. Only single recombinant antigens have been used previously as probes in this ICA and it was unclear whether the method was applicable to complex probes such as whole bacterial cells. Here, we describe the enrichment of plasmablasts specific for polysaccharide and protein antigens of both Streptococcus pneumoniae and Neisseria meningitidis using whole formalin-fixed bacterial cells as probes. The modified ICA protocol described here allowed for a pathogen-specific hmAb cloning efficiency of >80%. |
| Databáze: | OpenAIRE |
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