| Autor: |
Wolfgang J. Rettig, Pilar Garin-Chesa, Dieter Moosmayer, Dafne Müller, Elke Gerlach, Thomas Wüest, Michael Mersmann, Klaus Pfizenmaier, Alexej Schmidt |
| Rok vydání: |
2001 |
| Předmět: |
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| Zdroj: |
European Journal of Biochemistry. 268:1730-1738 |
| ISSN: |
0014-2956 |
| DOI: |
10.1046/j.1432-1327.2001.02046.x |
| Popis: |
Four completely human antibody derivatives [single-chain-antibody fragments (scFvs)] with specificity for the general tumor stroma marker fibroblast activation protein (FAP) were isolated by guided selection. Highly diverse IgG, IgM and IgD isotypes comprising heavy-chain variable domain libraries were generated using cDNAs derived from diverse lymphoid organs of a multitude of donors. Three of the human scFvs were converted into bivalent minibodies and expressed in eukaryotic cells for further functional characterization. Binding-competition studies and analysis by fluorescence-activated cell sorting showed high-affinity binding (10–20 nm) for two clones and recognition of the same epitope as the murine guiding antibody. The minibodies were successfully used for immunohistology of a variety of human carcinoma biopsies, revealing specific staining of stromal fibroblasts. Therefore, they should be suitable for in vivo diagnostic and tumor-targeting studies and, because of their completely human origin, be superior to murine or humanized antibody derivatives. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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