The Production of L–Phenylalanine by Polyazetidine Immobilized Microbes
| Autor: | Leon Lantz, John P. Hamman, Louis L. Wood, Mark Hampton Updike, Gary J. Calton |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
chemistry.chemical_classification
Biomedical Engineering Bioengineering Phenylalanine Biology medicine.disease_cause Applied Microbiology and Biotechnology Transaminase chemistry.chemical_compound Enzyme Biochemistry chemistry Oxaloacetic acid Aspartic acid medicine Molecular Medicine Amine gas treating Pyruvic acid Escherichia coli Biotechnology |
| Zdroj: | Nature Biotechnology. 4:317-320 |
| ISSN: | 1546-1696 1087-0156 |
| Popis: | Phenylalanine was produced from phenylpyruvate at up to 30 g/l in molar yields as high as 98% using polyazetidine immobilized E. coli high in aspartate phenylalanine transaminase. E. coli mutants containing only the tyrosine phenylalanine transaminase (tyrB) or the branched chain transaminase (ilvE) were unable to convert phenylpyruvate to phenylalanine at high levels. Aspartic acid was the amine donor of choice as the oxaloacetic acid produced in the reaction was readily decarboxylated to pyruvic acid under the experimental conditions thereby driving the reaction to completion. The catalyst had a projected half–life of greater than 8 months at temperatures of 37–41°C. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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