Identification of carotenoids and chlorophylls from green algae Chlorococcum humicola and extraction by liquefied dimethyl ether
| Autor: | Panatpong Boonnoun, Artiwan Shotipruk, Farahnaz Eghbali Babadi, Sorawit Powtongsook, Kasidit Nootong, Motonobu Goto |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
chemistry.chemical_classification Lutein Chromatography biology Chemistry General Chemical Engineering Extraction (chemistry) 04 agricultural and veterinary sciences biology.organism_classification 040401 food science 01 natural sciences Biochemistry Solvent chemistry.chemical_compound 0404 agricultural biotechnology Neoxanthin 010608 biotechnology Acetone Dimethyl ether Green algae Carotenoid Food Science Biotechnology |
| Zdroj: | Food and Bioproducts Processing. 123:296-303 |
| ISSN: | 0960-3085 |
| Popis: | In this study, the microalga Chlorococum humicula was evaluated as a potential source of carotenoids and chlorophylls, and extraction of these pigments with liquefied dimethyl ether (DME) as an alternative green solvent was investigated. Carotenoids and chlorophylls in the algae were identified by spectral and comparative band ratio analyses. The liquefied DME extraction was shown to be a rather fast process for the extraction of pigments from C. humicula at relatively high yields. The effects of the extraction solvent: sample ratio, time, and temperature in the liquefied DME extraction of C. humicola on the yields of neoxanthin, violaxanthin, lutein, chlorophyll-b, chlorophyll-a, as well as the total carotenoids and chlorophylls, were determined. The highest total carotenoids yield (4.14 mg/g dry weight algae) and total chlorophylls yield (8.45 mg/g dry weight algae) were obtained using a 45:1 (w/w) liquefied DME: algae wet weight ratio for 20 min at 41 °C. At this condition, extraction of 91 % and 35 % of the total carotenoids and chlorophylls, respectively, was attained. In contrast, the extraction yield and percentage recovery of carotenoids obtained with acetone, a conventional solvent, was found to be lower than that of the liquefied DME, suggesting that liquefied DME has a higher selectivity for the less polar carotenoids than that of acetone. |
| Databáze: | OpenAIRE |
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