Characterization of partial NS5B region among hepatitis C virus genotype 6 subtypes isolated from Thai blood donors
| Autor: | Suriya Chaiwong, Anchalee Sistayanarain |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Genetics Phylogenetic tree Hepatitis C virus 030106 microbiology virus diseases Biology medicine.disease_cause Virology digestive system diseases Amino acid 03 medical and health sciences chemistry.chemical_compound Infectious Diseases chemistry Hepatitis C virus genotype Genotype medicine Gene NS5B Nested polymerase chain reaction |
| Zdroj: | Journal of Medical Virology. 88:1785-1790 |
| ISSN: | 0146-6615 |
| Popis: | The hepatitis C virus (HCV) is an important cause of liver dysfunction which continues to spread in Thailand, particularly as genotype 6. The NS5B gene fragment is particularly variable and thus provides a valuable tracker for its spread. Therefore, the purpose of this study was to characterize the HCV genotype 6 based on partial NS5B region using Thai blood donor samples. Twenty-nine samples were genotyped as HCV 6 by nested PCR, nucleotide sequencing and amino acid sequence analysis. Amplified products were identified as HCV genotypes 6f, 6c, 6n, and 6i. There were amino acid variations of 4-18 residues in subtypes 6f, 6c, and 6n whereas subtype 6i was conserved when compared with their referent strains. In subtypes 6f, 6c, 6n, and 6i, the amino acid mutations at positions 244, 309, and 310 which are associated with HCV resistance were present. In summary, the sequences and phylogenetic analysis of NS5B of HCV used in our study yielded the genotypes 6f, 6c, 6n, and 6i. This finding indicates diversity of amino acids in NS5B of HCV. Characterizing the partial NS5B region among hepatitis C virus genotype 6 subtypes may predict efficacious anti-HCV therapy. J. Med. Virol. 88:1785-1790, 2016. © 2016 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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