SUCCESSFUL SEPARATION AND DETERMINATION OF ISOMERS OF CYTOSINE DERIVATIVES FOR HPLC

Autor:	Aneta Kondrzycka-Dąda, Mariusz Kluska, Dorota Prukała, Konrad Małkiewicz, Magdalena Komasińska, Wiesław Prukała
Rok vydání:	2014
Předmět:	Chromatography Formic acid Aryl Clinical Biochemistry Pharmaceutical Science Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound chemistry Phase (matter) Methanol Acetonitrile Selectivity Cytosine
Zdroj:	Journal of Liquid Chromatography & Related Technologies. 37:2172-2181
ISSN:	1520-572X 1082-6076
DOI:	10.1080/10826076.2013.828304
Popis:	The work presents research results on separation and determination of three isomers: 1-N-o-(m- or p-)bromobenzyl-[5-(4′-morpholinyl)methyl]cytosines. The research included three stationary phases and three mobile phases: acetonitrile, methanol, mixture of acetonitrile/water/formic acid. When using octadecyl stationary phase considered to be standard as well as the octyl one, no separation of the aforementioned isomers of cytosine derivatives was observed, regardless of the mobile phase type. It was only when the chemically bonded aryl (naphthylpropyl) stationary phase was used that the separation of the aforementioned isomers was possible within 7 min. This phase was also characterized by the greatest selectivity (α = 1.90 and 1.53) of the cytosine derivatives being assayed, with the use of methanol as the mobile phase at 0.5 mL/min flow.
Databáze:	OpenAIRE
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