Immobilization in Nanomatrices of Humicola Lutea Mycelium for Alpha-Galactosidase Biosynthesis in Laboratory Air-Lift Bioreactor

Autor:	D. Spasova, Lilyana Nacheva, L. Nacheva, P. Aleksieva, P. Djambaski, Georgi Chernev
Rok vydání:	2010
Předmět:	chemistry.chemical_classification biology Scanning electron microscope Enzyme assay Lactic acid Sepharose chemistry.chemical_compound Enzyme chemistry Biochemistry biology.protein Bioreactor Fermentation Mycelium Biotechnology Nuclear chemistry
Zdroj:	Biotechnology & Biotechnological Equipment. 24:1897-1903
ISSN:	1314-3530 1310-2818
DOI:	10.2478/v10133-010-0041-3
Popis:	The sol-gel synthesis of both hybrid nanomatrices containing tetraethylortosilicate (TEOS) as an inorganic precursor and lactic acid, or sepharose as an organic component was made. Crystal as well as surface morphology of the hybrids were investigated using different methods: X-ray diffraction, infrared spectra, BET-analysis and atomic force microscopy (AFM). The obtained nanomatrices were applicated for immobilization of the α-galactosidase producing fungal strain Humicola lutea 120-5. The semicontinuous cultivation was carried out in laboratory air-lift bioreactor. Maximal level of enzyme activity (1050-1130 U/l) that was reached in the third to fourth fermentation cycle using TEOS+40% lactic acid was higher than that obtained in the samples with TEOS+20% sepharose (660-770 U/l). The correlation between enzyme productivity and fungal development in the pore structure of the carriers was examined using scanning electron microscopy observation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fd97c8a70b70bd3b67c35a8ea77773ab https://doi.org/10.2478/v10133-010-0041-3 Zobrazit plný text záznamu