| Autor: |
A.‐K. Fentz, Minka Breloer, Bernhard Fleischer, A von Bonin, Solveig H. Moré |
| Rok vydání: |
2002 |
| Předmět: |
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| Zdroj: |
Scandinavian Journal of Immunology. 55:329-335 |
| ISSN: |
0300-9475 |
| DOI: |
10.1046/j.1365-3083.2002.01061.x |
| Popis: |
Lipopolysaccharide (LPS) can activate human and murine T cells in vivo and in vitro. Here we analysed the effects of LPS on T cells with defined specificities in T-cell receptor (TCR)-transgenic systems. LPS rapidly induced high amounts of interferon (IFN)-y in a subpopulation of purified T cells from DOI 1.10 (OVA 3 2 3 - 3 3 9 /H2-A d ) and OT-1 (OVA 2 5 7 - 2 6 4 /H2-K b ) mice when coincubated with antigen-pulsed peritoneal exudate cells (PECs). LPS induced IFN-γ in T cell cultures even when the number of antigenic major histocompatibility complex (MHC) class-I complexes was too small to stimulate the T cells. LPS, thus, overruled the unresponsiveness of the otherwise 'antigen-ignorant' T cells. The release of IFN-y strictly correlates with the PECs' ability to produce interleukin (IL)-12. In contrast to the induction of IFN-y, antigen-specific IL-2 secretion and proliferation of T cells were rather decreased in the presence of LPS. Only very few IFN-γ-secreting natural killer (NK) cells and natural killer T (NKT) cells in the given experimental system could be detected using intracellular fluorescence-activated cell sorter (FACS) staining. Taken together, our results indicate that LPS has the potential to activate quiescent T cells and to specifically induce IFN-y in CD4 and CD8 T cells. This may have direct consequences for the activation of autoreactive T cells following bacterial infections. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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